E.Z.N.A.? Gel Extraction Kit 15分鐘內從瓊脂糖凝膠中回收DNA片段

從瓊脂糖凝膠中回收純化特定的DNA片段是一種很常規的技術(shù)，但是大多數的方法要么不能完全去除瓊脂糖（瓊脂糖對下游操作影響很大），要么會(huì )出現DNA被剪斷或者得率很低的情況。

E.Z.N.A? Gel Extraction Kit采用硅膠柱純化技術(shù)，適合從各種類(lèi)型各種濃度的瓊脂糖凝膠中回收100bp-20kb的DNA片段，回收率可達到60-70%，可直接應用于酶促連接反應、PCR擴增、酶切以及標記等實(shí)驗。

此外，該試劑盒還可以用從于PCR產(chǎn)物，酶切產(chǎn)物中直接純化DNA。將目的DNA片段從瓊脂糖凝膠中切下，加入Binding Buffe水浴溶解凝膠，而后轉移至HiBind?離心柱上離心結合DNA，經(jīng)兩步簡(jiǎn)單洗滌，用Elution Buffer洗脫DNA。

產(chǎn)品特性與優(yōu)點(diǎn) 快速--可以在15分鐘內完成純化工作 可靠--緩沖液保證DNA純度和高回收率 安全--無(wú)須接觸有害的有機物抽提 高質(zhì)--純化的DNA適合于各種應用 片段大--可回收大于10kb的DNA片段

送硅膠柱、 離心管 等耗材各一包.

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Gel purification of DNA is a common technique used for the isolation of specific DNA fragments from reaction products. However, most methods either fail to completely remove agarose, which can lead to problems in downstream manipulations; shear the DNA; or result in very low yields. The E.Z.N.A.? Gel Extraction kit uses HiBind DNA spin-column technology to purify DNA bands 70 bp – 20 kb in length from all grades of agarose gels with up to 85% recovery. The DNA band of interest is excised from the gel, dissolved in binding buffer, and applied to a DNA HiBind spin column. Following three wash steps, DNA is eluted with deionized water or elution buffer and is ready for downstream applications such as ligations, PCR amplification, restriction enzyme digestion, and various labeling reactions. This kit can be also used to purify DNA fragments from PCR products and enzymatic reactions. Two different spin column formats (V-Spin column and Q-Spin Column) are available for this kit.

• Rapid – DNA recovery from agarose gel in 15 min
• Reliable – Optimized buffers guarantee pure DNA
• High-quality – Purified DNA suitable for most applications

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